Ancient DNA [electronic resource] : methods and protocols / edited by Beth Shapiro, Michael Hofreiter.

Bibliographic Details
Uniform Title:Methods in molecular biology (Clifton, N.J.) ; v. 840.
Springer protocols.
Other Authors: Shapiro, Beth Alison
Hofreiter, Michael, 1973-
Language:English
Published: New York : Humana Press ; Springer, c2012.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 840.
Springer protocols.
Subjects:
DNA, Fossil > Analysis > Methodology.
Animals, Fossil > Genetics.
Extinct animals > Genetics.
Online Access:
Springer Nature Complete Protocols: 2012 (Springer Link)
Variant Title:
Ancient DNA: Methods and Protocols
Format: Electronic eBook
Contents:
  • Setting up an ancient DNA laboratory / Tara L. Fulton
  • A phenol-chloroform protocol for extracting DNA from ancient samples / Ross Barnett and Greger Larson
  • DNA extraction of ancient animal hard tissue samples via adsorption to silica particles / Nadin Rohland
  • Case study : recovery of ancient nuclear DNA from toe pads of the extinct passenger pigeon / Tara L. Fulton, Stephen M. Wagner, and Beth Shapiro
  • Extraction of DNA from paleofeces / Melanie Kuch and Hendrik Poinar
  • DNA extraction from keratin and chitin / Paula F. Campos and Thomas M.P. Gilbert
  • Case study : ancient sloth DNA recovered from hairs preserved in paleofeces / Andrew A. Clack, Ross D.E. MacPhee, and Hendrik N. Poinar
  • Ancient DNA extraction from soils and sediments / James Haile
  • DNA extraction from fossil eggshell / Charlotte L. Oskam and Michael Bunce
  • Ancient DNA extraction from plants / Logan Kistler
  • DNA extraction from formalin-fixed material / Paula F. Campos and Thomas M.P. Gilbert
  • Case study : ancient DNA recovered from Pleistocene-age remains of a Florida armadillo / Brandon Letts and Beth Shapiro
  • Nondestructive DNA extraction from museum specimens / Michael Hofreiter
  • Case study : using a nondestructive DNA extraction method to generate mtDNA sequences from historical chimpanzee specimens / Elmira Mohandesan, Stefan Prost, and Michael Hofreiter
  • PCR amplification, cloning, and sequencing of ancient DNA / Tara L. Fulton and Mathias Stiller
  • Quantitative real-time PCR in aDNA research / Michael Bunce, Charlotte L. Oskam, and Morten E. Allentoft
  • Multiplex PCR amplification of ancient DNA / Mathias Stiller and Tara L. Fulton
  • Preparation of next-generation sequencing libraries from damaged DNA / Adrian W. Briggs and Patricia Heyn
  • Generating barcoded libraries for multiplex high-throughput sequencing / Michael Knapp, Mathias Stiller, and Matthias Meyer
  • Case study : targeted high-throughput sequencing of mitochondrial genomes from extinct cave bears via direct multiplex PCR sequencing (DMPS) / Mathias Stiller
  • Target enrichment via DNA hybridization capture / Susanne Horn
  • Case study : enrichment of ancient mitochondrial DNA by hybridization capture / Susanne Horn
  • Analysis of high-throughput ancient DNA sequencing data / Martin Kircher
  • Phylogenetic analysis of ancient DNA using BEAST / Simon Y.W. Ho.