T cell protocols / edited by Gennaro De Libero.

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Bibliographic Details
Uniform Title:Methods in molecular biology (Clifton, N.J.) ; v. 514.
Springer protocols (Series)
Other Authors: De Libero, Gennaro
Language:English
Published: New York : Humana Press, [2009], ©2009.
Edition:Second edition.
Series:Methods in molecular biology (Clifton, N.J.) ; v. 514.
Springer protocols (Series)
Subjects:
T-Lymphocytes > immunology.
Laboratory Manual.
T cells > Laboratory manuals.
Genre:
Laboratory manuals.
Laboratory Manual.
Physical Description:xi, 165 pages, 3 unnumbered pages of plates : illustrations (some color) ; 27 cm.
Format: Book
Contents:
  • Analysis of frequency and phenotype of antigen-specific T cells / Angus Stock and Vincenzo Cerundolo
  • B cell helper assays / Sergio Abrignani, ... [et al.]
  • Transkingdom RNA interference (tkRNAi) / Thu A. Nguyen and Johannes H. Fruehauf
  • Flow cytometry and cell activation / Sonia Gavasso
  • Investigating T cells by polychromatic flow cytometry / Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza
  • Generation of human T cell clones / Sabrina Mariotti and Roberto Nisini
  • Limiting dilution analysis of antigen-specific T cells / Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan
  • T cell epitope-mapping by cytokine gene expression assay / Maurizio Provengano and Giulio C. Spagnoli
  • Cytokine multiplex immunoassay / Wilco de Jager, Berent Prakken, and Ger T. Rijkers
  • Purification of the T cell antigen receptor and analysis by blue-native PAGE / Mahima Swamy and Wolfgang W.A. Schamel
  • Non-replicating recombinant vaccinia virus expressing CD80 to enhance T-cell stimulation / Paul Zajac.