Human cell culture protocols [electronic resource] / edited by Ragai R. Mitry, Robin D. Hughes.
Uniform Title: | Methods in molecular biology (Clifton, N.J.) ;
v. 806. Springer protocols. |
---|---|
Other Authors: | |
Language: | English |
Published: |
New York :
Humana Press,
c2012.
|
Edition: | 3rd ed. |
Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 806. Springer protocols. |
Subjects: | |
Genre: | |
Online Access: | |
Format: | Electronic eBook |
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245 | 0 | 0 | |a Human cell culture protocols |h [electronic resource] / |c edited by Ragai R. Mitry, Robin D. Hughes. |
250 | |a 3rd ed. | ||
260 | |a New York : |b Humana Press, |c c2012. | ||
490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 806 | |
490 | 1 | |a Springer protocols | |
504 | |a Includes bibliographical references and index. | ||
505 | 0 | |a 1. Introduction to cell culture / Christina Philippeos, Robin D. Hughes, Anil Dhawan and Ragai R. Mitry -- 2. Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes / Ling Li, Mizubo Fukunaaga -- 3. Isolation, cultivation, and application of human alveolar epithelial cells / Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr -- 4. Culture of parathyroid cells / Peyman Björklund and Per Hellman -- 5. Functional analysis of human islets of langerhans maintained in culture / Shanta J. Persaud, Bo Liu, and Peter M. Jones -- 6. Conversion of non-endocrine human pancreatic cells to insulin-producing cells for treatment of diabetes / Min Zhao and Guo Cai Huang -- 7. Evaluation of cytochrome P450 activities in human hepatocytes in vitro / María José Gómez-Lechón, Agustín Lahoz, José V. Castell, and María Teresa Donato -- 8. Human hepatocytes: isolation, culture, and quality procedures / Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andrea K. Nussler -- 9. Preclinical testing ov virotherapeutics for primary and secondary tumors of the liver / Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer -- 10. Isolation and functional studies of human fetal gastric epithelium in primary culture / Pierre Chailler, Jean-Franc̦ois Beaulieu, and Daniel Ménard -- 11. Isolation, characterization, and culture of normal human intestinal crypt and villus cells / Jean-Franc̦ois Beaulieu and Daniel Ḿenard -- 12. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts / Claire C. Sharpe and Mark E.C. Dockrell -- 13. Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart -- 14. Culture of isolated human adipocytes and isolated adipose tissue / Kirstin A. Carwswell, Mi-Jeong Lee, and Susan K. Fried -- 15. Primary culture of human adipocyte precursor cells: expansion and differentiation / Thomas Skurk and Hans Hauner -- 16. Primary culture of ovarian cells for research on cell interactions in the hormonal control of steroidogenesis / Jerzy F. Galas -- 17. Human vascular smooth muscle cell culture / Diane Proudfoot and Catherine Shanahan -- 18. Culture of human endothelial cells from umbilical veins / Richard C.M. Siow -- 19. Human peripheral blood mononuclear cell culture for flow cytometric analysis of phosphorylated mitogen-activated protein kinases / Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos -- 20. Human CD4+CD25(high [superscript]) CD127 (low/neg [superscript]) regulatory T cells / Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma -- 21. Human chondrocyte cultures as models of cartilage-specific gene regulation / Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring -- 22. Isolation and culture of human osteoblasts / Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher -- 23. Human osteoclast culture and phenotypic characterization / Ankita Agrawal, James A. Gallagher, and Alison Gartland -- 24. Effects of temperature generated from the holmium: Yag laser on human osteoblasts in monolayer tissue culture / Moustafa I. Hafez, Anne Sandison, Richard R. H. Coombs, Ian D. McCarthy, and Al-Shymaa M. Hafez -- 25. Laser microdissection microscopy: application to cell culture / Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia -- 26. Automated adherent human cell culture (mesenchymal stem cells) / Robert Thomas and Elizabeth Ratcliffe -- 27. Culturing and differentiating human mesenchymal stem cells for biocompatible scaffolds in regenerative medicine / William R. Otto and Catherine E. Sarraf. | |
650 | 0 | |a Human cell culture |v Laboratory manuals. | |
700 | 1 | |a Mitry, Ragai R. | |
700 | 1 | |a Hughes, Robin D. | |
773 | 0 | |t Springer Nature Complete Protocols |d Springer Nature | |
776 | 1 | |t Human cell culture protocols / |w (OCoLC)ocn751802917 |w (DLC)2011939785 | |
830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 806. | |
830 | 0 | |a Springer protocols. | |
856 | 4 | 0 | |y Access Content Online(from Springer Nature Complete Protocols) |u https://ezproxy.msu.edu/login?url=https://link.springer.com/10.1007/978-1-61779-367-7 |z Springer Nature Complete Protocols: 2012 |