Plant fungal pathogens [electronic resource] : methods and protocols / edited by Melvin D. Bolton, Bart P.H.J. Thomma.
| Uniform Title: | Methods in molecular biology (Clifton, N.J.) ;
v. 835. Springer protocols. |
|---|---|
| Other Authors: | |
| Language: | English |
| Published: |
New York :
Humana Press,
c2012.
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 835. Springer protocols. |
| Subjects: | |
| Online Access: | |
| Variant Title: |
Plant Fungal Pathogens: Methods and Protocols |
| Format: | Electronic eBook |
MARC
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| 245 | 0 | 0 | |a Plant fungal pathogens |h [electronic resource] : |b methods and protocols / |c edited by Melvin D. Bolton, Bart P.H.J. Thomma. |
| 246 | 2 | |a Plant Fungal Pathogens: Methods and Protocols | |
| 260 | |a New York : |b Humana Press, |c c2012. | ||
| 490 | 1 | |a Methods in molecular biology, |x 1940-6029 ; |v 835 | |
| 490 | 1 | |a Springer protocols | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a Fungal ABC transporter deletion and localization analysis -- Targeted gene replacement in fungal pathogens via agrobacterium tumefaciens- mediated transformation -- Activity-based protein profiling of infected plants -- The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in nicotiana benthamiana leaves -- Proteomic techniques for plant-fungal interactions -- Identification of HR-inducing cDNAs from plant pathogens via a Gateway((r))-compatible binary potato virus X-expression vector -- Freeze-fracture of infected plant leaves in ethanol for scanning electron microscopic study of fungal pathogens -- Real-time PCR for the quantification of fungi in planta -- Using non-homologous end-joining-deficient strains for functional gene analyses in filamentous fungi -- Atomic force microscopy: A tool for studying biophysical surface properties underpinning fungal interactions with plants and substrates -- Use of the yeast two-hybrid system to identify targets of fungal effectors -- Characterization of plant-fungal interactions involving necrotrophic effector-producing plant pathogens -- Heterologous production of fungal effectors in pichia pastoris -- The application of laser microdissection to profiling fungal pathogen gene expression in planta -- Metabolomics protocols for filamentous fungi -- Targeted gene replacement in fungi using a split-marker approach -- Massively parallel sequencing technology in pathogenic microbes -- Confocal microscopy in plant-pathogen interactions -- The use of open source bioinformatics tools to dissect transcriptomic data -- Population biology of fungal plant pathogens -- Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens -- In vitro induction of infection-related hyphal structures in plant pathogenic fungi -- Fungicide resistance assays for fungal plant pathogens -- Identification of lipid-binding effectors -- In silico identification and characterization of effector catalogs -- Horizontal transfer of supernumerary chromosomes in fungi -- The induction of mycotoxins by trichothecene producing fusarium species -- Identifying genes in fusarium verticillioides through forward and reverse genetics -- Assessment of autophagosome formation by transmission electron microscopy -- Fungal plant pathogen detection in plant and soil samples using DNA macroarrays -- Random insertional mutagenesis in fungal genomes to identify virulence factors -- A yeast secretion trap assay for identification of secreted proteins from eukaryotic phytopathogens and their plant hosts -- Comparing fungal genomes: Insight into functional and evolutionary processes -- Multigene phylogenetic analyses to delimit new species in fungal plant pathogens -- MAP kinase phosphorylation and cAMP assessment in fungi -- A one-step affinity-purification protocol to purify NB-LRR immune receptors from plants that mediate resistance to fungal pathogens -- Karyotyping methods for fungi -- Isolation of apoplastic fluid from leaf tissue by the vacuum infiltration-centrifugation technique -- Gene cloning using degenerate primers and genome walking -- Construction of hairpin RNA-expressing vectors for RNA-mediated gene silencing in fungi -- An unbiased method for the quantitation of disease phenotypes using a custom-built macro plugin for the program imageJ. | |
| 650 | 0 | |a Plant-pathogen relationships. | |
| 650 | 0 | |a Fungal diseases of plants. | |
| 700 | 1 | |a Bolton, Melvin D. |q (Melvin Dennis), |d 1977- | |
| 700 | 1 | |a Thomma, Bart P. H. J. | |
| 773 | 0 | |t Springer Nature Complete Protocols |d Springer Nature | |
| 776 | 1 | |t Plant fungal pathogens |w (OCoLC)ocn752068533 |w (DLC)2011943884 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 835. | |
| 830 | 0 | |a Springer protocols. | |
| 856 | 4 | 0 | |y Access Content Online(from Springer Nature Complete Protocols) |u https://ezproxy.msu.edu/login?url=https://link.springer.com/10.1007/978-1-61779-501-5 |z Springer Nature Complete Protocols: 2012 |