Dengue [electronic resource] : methods and protocols / edited by Radhakrishnan Padmanabhan, Department of Micobiology and Immunology, Georgetown University, Washington, DC, USA, Subhash G. Vasudevan, Emerging Infectious Diseases Program, Duke-NUS Gradute Medical School, Singapore, Singapore.

"Includes cutting-edge methods and protocols -- Provides step-by-step detail essential for reproducible results -- Contains key notes and implementation advice from the experts -- Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encep...

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Bibliographic Details
Uniform Title:Methods in molecular biology (Clifton, N.J.) ; v. 1138. 1064-3745
Springer protocols (Series) 1949-2448
Other Authors: Padmanabhan, Radhakrishnan (Editor)
Vasudevan, Subhash (Editor)
Language:English
Published: New York, NY : Humana Press, [2014]
Series:Methods in molecular biology (Clifton, N.J.) ; v. 1138.
Springer protocols (Series)
Subjects:
Dengue > Laboratory manuals.
Virology > Technique.
Medical virology > Laboratory manuals.
Antiviral agents.
Genre:
Laboratory manuals.
Online Access:
Springer Nature Complete Protocols: 2014 (Springer Link)
Variant Title:
Dengue: Methods and Protocols
Format: Electronic eBook
Partial Contents:
  • Dengue Virus Growth, Purification and Fluorescent Labeling
  • Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique
  • Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique
  • Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy
  • Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion
  • Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection
  • Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging
  • Development and Application of Dengue Virus Reverse Genetic Systems
  • DENV4 Renilla Luciferase Reporter Replicons
  • Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination
  • Identification of Dengue-Specific Human Antibody Fragments Using Phage Display
  • Next Generation Whole Genome Sequencing of Dengue Virus
  • MPGAfold in Dengue Secondary Structure Prediction
  • Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE
  • Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5
  • Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry
  • Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore
  • Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation
  • Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import
  • Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening
  • Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors
  • Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase
  • Animal Models in Dengue
  • Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model.