Cryotechniques in Biological Electron Microscopy [electronic resource] edited by Rudolf A. Steinbrecht, Karl Zierold.

To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more...

Full description

Bibliographic Details
Corporate Author:	SpringerLink (Online service)
Other Authors:	Steinbrecht, Rudolf A. (Editor) Zierold, Karl (Editor)
Language:	English
Published:	Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1987.
Edition:	1st ed. 1987.
Subjects:	Cytology. Biophysics. Anatomy.
Online Access:	Springer Book Archive - Biomedicine & Life Sciences: 1987 (Springer Link)
Format:	Electronic eBook

Contents:

I Fundamentals
1 Physics of Water and Ice: Implications for Cryofixation
2 The Response of Biological Macromolecules and Supramolecular Structures to the Physics of Specimen Cryopreparation
3 Electron Beam Radiation Damage to Organic and Biological Cryospecimens
II General Methodology
4 Cryofixation Without Pretreatment at Ambient Pressure
5 Cryoeleetron Microscopy of Vitrified Specimens
6 Cryoultramicrotomy
7 Freeze-Substitution and Freeze-Drying
III Special Aspects
8 Theory and Practice of High Pressure Freezing
9 Freeze-Etching of Dispersions, Emulsions and Macromolecular Solutions of Biological Interest 192
10 High Resolution Metal Replication of Freeze-Dried Specimens
11 Immunogold Labelling of Cryosections and Cryofractures
12 Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistry
13 Scanning Electron Microscopy and X-Ray Microanalysis of Frozen-Hydrated Bulk Samples
14 Cryofixation of Dynamic Processes in Cells and Organelles
15 Cryofixation of Diffusible Elements in Cells and Tissues for Electron Probe Microanalysis
IV Appendix
16 Safety Rules for Cryopreparation.